Western blotting analysis using dystroglycan 1 antibody (Cat#61890). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dystroglycan 1 antibody (Cat#61890, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using dystroglycan 1 antibody (Cat#61890). Dystroglycan 1 expression in wild-type (WT) and dystroglycan 1 (DAG1) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dystroglycan 1 antibody (Cat#61890, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
A synthesized peptide derived from human beta Dystroglycan
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000
Note
This product is for research use only.
Data
Western blotting analysis using dystroglycan 1 antibody (Cat#61890). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dystroglycan 1 antibody (Cat#61890, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using dystroglycan 1 antibody (Cat#61890). Dystroglycan 1 expression in wild-type (WT) and dystroglycan 1 (DAG1) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dystroglycan 1 antibody (Cat#61890, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using dystroglycan 1 antibody (Cat#61890). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dystroglycan 1 antibody (Cat#61890, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using dystroglycan 1 antibody (Cat#61890). Dystroglycan 1 expression in wild-type (WT) and dystroglycan 1 (DAG1) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dystroglycan 1 antibody (Cat#61890, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000818s ]
SELECT * FROM `goods` WHERE `code` = '61890' LIMIT 1 [ RunTime:0.000492s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000405s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000351s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000365s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 7763 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000354s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000526s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 7763 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000305s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000437s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000259s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.054913s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8234,8964,5001,3910,10517,5986,4363,4084,8445,5413) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000828s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000455s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000203s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.005072s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000257s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000335s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.002854s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000247s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000236s ]
