Western blotting analysis using epidermal growth factor receptor antibody (Cat#63472). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with epidermal growth factor receptor antibody (Cat#63472, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using epidermal growth factor receptor antibody (Cat#63472). Epidermal growth factor receptor expression in wild type (WT) and epidermal growth factor receptor (EGFR) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with epidermal growth factor receptor antibody (Cat#63472, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Epidermal growth factor receptor expression in HepG2 cells using Epidermal growth factor receptor antibody (Cat#63472, 1:2,000). Green, isotype control; red, Epidermal growth factor receptor.
Validation of Epidermal growth factor receptor knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with Epidermal growth factor receptor antibody (Cat#63472, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HepG2 cells with Epidermal growth factor receptor antibody (Cat#63472, 1:1,000) . Nuclei were stained blue with DAPI; Epidermal growth factor receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Low. Scale bar, 20 μm.
Immunocytochemical staining of HepG2 cells using Epidermal growth factor receptor antibody (Cat#63472, 1:1,000), Top panel: wild-type (WT); Bottom panal: Adiponectin receptor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Epidermal growth factor receptor was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
