Western blotting analysis using IQGAP1 antibody (Cat#62130). IQGAP1 expression in wild-type (WT) and IQGAP1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IQGAP1 antibody (Cat#62130, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
IQGAP1; IQ Motif Containing GTPase Activating Protein 1; P195; HUMORFA01; KIAA0051; SAR1; Ras GTPase-Activating-Like Protein IQGAP1; RasGAP-Like With IQ Motifs
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using IQGAP1 antibody (Cat#62130). IQGAP1 expression in wild-type (WT) and IQGAP1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IQGAP1 antibody (Cat#62130, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using IQGAP1 antibody (Cat#62130). IQGAP1 expression in wild-type (WT) and IQGAP1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with IQGAP1 antibody (Cat#62130, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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