Western blotting analysis using caspase 3 antibody (Cat#63400). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 3 antibody (Cat#63400, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using caspase 3 antibody (Cat#63400). Caspase 3 expression in wild-type (WT) and caspase 3 (CASP3) shRNA knockdown (KD) HAP-1 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with caspase 3 antibody (Cat#63400, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using caspase 3 antibody (Cat#63400, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
