Western blotting analysis using anti-phospho-CREB (S133) antibody (Cat#R0019). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-CREB (S133) antibody (Cat#R0019, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-CREB (S133) expression in C2C12 cells using anti-Phospho-CREB (S133) antibody (Cat#R0019, 1:2,000). Green, isotype control; red, Phospho-CREB (S133).
Immunocytochemical staining of C2C12 cells with anti-Phospho-CREB (S133) antibody (Cat#R0019, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-CREB (S133) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low . Scale bar, 20 μm.
Western blotting analysis using anti-phospho-CREB (S133) antibody (Cat#R0019). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-CREB (S133) antibody (Cat#R0019, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-CREB (S133) expression in C2C12 cells using anti-Phospho-CREB (S133) antibody (Cat#R0019, 1:2,000). Green, isotype control; red, Phospho-CREB (S133).
Immunocytochemical staining of C2C12 cells with anti-Phospho-CREB (S133) antibody (Cat#R0019, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-CREB (S133) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low . Scale bar, 20 μm.
Western blotting analysis using anti-phospho-CREB (S133) antibody (Cat#R0019). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-CREB (S133) antibody (Cat#R0019, 1:2,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Phospho-CREB (S133) expression in C2C12 cells using anti-Phospho-CREB (S133) antibody (Cat#R0019, 1:2,000). Green, isotype control; red, Phospho-CREB (S133).
Immunocytochemical staining of C2C12 cells with anti-Phospho-CREB (S133) antibody (Cat#R0019, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-CREB (S133) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Low . Scale bar, 20 μm.
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