Western blotting analysis using ARHGDIA antibody (Cat#63501). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARHGDIA antibody (Cat#63501, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ARHGDIA antibody (Cat#63501). ARHGDIA expression in wild type (WT) and ARHGDIA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ARHGDIA antibody (Cat#63501, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with ARHGDIA antibody (Cat#63501, 1:1,000). Nuclei were stained blue with DAPI; ARHGDIA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Flow cytometric analysis of ARHGDIA expression in HepG2 cells using ARHGDIA antibody (Cat#63501, 1:2,000). Green, isotype control; red, ARHGDIA.
