Western blotting analysis using HADHA antibody (Cat#63587). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HADHA antibody (Cat#63587). HADHA expression in wild type (WT) and HADHA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using HADHA antibody (Cat#63587, 1:1,000), Top panel: wild-type (WT); Bottom panal: HADHA shRNA knockdown (KD). Nuclei were stained blue with DAPI; HADHA was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of HADHA expression in HepG2 cells using HADHA antibody (Cat#63587, 1:2,000). Green, isotype control; red, HADHA.
Validation of HADHA knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HADHA antibody (Cat#63587, 1:2,000) and analyzed using BD flow cytometer.
