Western blotting analysis using ring-box 1 antibody (Cat#63745). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ring-box 1 antibody (Cat#63745, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ring-box 1 antibody (Cat#63745). Ring-box 1 expression in wild-type (WT) and ring-box 1 (RBX1) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ring-box 1 antibody (Cat#63745, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with ring-box 1 antibody (Cat#63745, 1:1,000) . Nuclei were stained blue with DAPI;Ring-box 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Scale bar, 20 μm.
Flow cytometric analysis of ring-box 1 expression in C2C12 cells using Ring-box 1 antibody (Cat#63745, 1:2,000). Green, isotype control; red, Ring-box 1.
Validation of Ring-box 1 knockdown using flow cytometry. Wild-type(WT, Blue) and Ring-box 1 knockdown(KD, Green) HT-1080 cells were stained with Ring-box 1 antibody(Cat#63745, 1:2,000) and analyzed using BD flow cytometer.
