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  • Primary Antibody
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Home>Product>Primary Antibody

KD-Validated Annexin A3 Mouse mAb

#66366

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shRNA Knockdown Validated
Western blotting analysis using annexin A3 antibody (Cat#66366). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with annexin A3 antibody (Cat#66366, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using annexin A3 antibody (Cat#66366). Annexin A3 expression in wild-type (WT) and annexin A3 (ANXA3) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with annexin A3 antibody (Cat#66366, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with annexin A3 antibody (Cat#66366, 1:1,000) . Nuclei were stained blue with DAPI; Annexin A3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL，100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    ANXA3
  • Catalog：
    66366
  • Application：
    WB, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Annexin A3 Mouse mAb
Aliases ANXA3; Annexin A3; ANX3; Inositol 1,2-Cyclic Phosphate 2-Phosphohydrolase; Placental Anticoagulant Protein III; 35-Alpha Calcimedin; Lipocortin III; Annexin-3; PAP-III; Annexin III (Lipocortin III, 1,2-Cyclic-Inositol-Phosphate Phosphodiesterase, Placental Anticoagulant Protein III, Calcimedin 35-Alpha); Annexin III (Lipocortin III); Calcimedin 35-Alpha; Annexin III
Background

Gene Name: ANXA3

NCBI Gene Entry: 306

UniProt Entry: P12429

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 31 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8705

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human ANXA3
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using annexin A3 antibody (Cat#66366). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with annexin A3 antibody (Cat#66366, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using annexin A3 antibody (Cat#66366). Annexin A3 expression in wild-type (WT) and annexin A3 (ANXA3) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with annexin A3 antibody (Cat#66366, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with annexin A3 antibody (Cat#66366, 1:1,000) . Nuclei were stained blue with DAPI; Annexin A3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    ANXA3
  • Catalog：
    66366
  • Application：
    WB, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using annexin A3 antibody (Cat#66366). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with annexin A3 antibody (Cat#66366, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using annexin A3 antibody (Cat#66366). Annexin A3 expression in wild-type (WT) and annexin A3 (ANXA3) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with annexin A3 antibody (Cat#66366, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with annexin A3 antibody (Cat#66366, 1:1,000) . Nuclei were stained blue with DAPI; Annexin A3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
  • Phone Number

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  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

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All Rights Reserved.

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  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
  146. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_param.php ( 0.75 KB )
  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
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  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  1. CONNECT:[ UseTime:0.000566s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000952s ]
  3. SELECT * FROM `goods` WHERE `code` = '66366' LIMIT 1 [ RunTime:0.000508s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000414s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000376s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000451s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 10314 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000379s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000431s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 10314 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000276s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000370s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000258s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.052676s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (137,8204,5218,3667,5611,7056,5821,5396,4155,4267) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000835s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000513s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000261s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000549s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000290s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000335s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000577s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000456s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000249s ]
0.093835s