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  • Primary Antibody
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Home>Product>Primary Antibody

Hypoxia Inducible Factor 1 Subunit Alpha Recombinant Rabbit mAb

#9321

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Recombinant Antibody
Western blotting analysis using hypoxia inducible factor 1 subunit alpha antibody (Cat#9321). Total cell lysates (30 μg) from various cell lines untreated or treated with cobalt chloride (250 μM, 6 hr) were loaded and separated by SDS-PAGE. The blot was incubated with hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat.#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of hypoxia inducible factor 1 subunit alpha expression in HT-1080 cells using hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:2,000). Green, isotype control; red, hypoxia inducible factor 1 subunit alpha.
Immunocytochemical staining of HT-1080 cells with Hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:1,000). Nuclei were stained blue with DAPI; Hypoxia inducible factor 1 subunit alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    HIF1A
  • Catalog：
    9321
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Hypoxia Inducible Factor 1 Subunit Alpha Recombinant Rabbit mAb
Aliases Hypoxia Inducible Factor 1 Subunit Alpha; Hypoxia Inducible Factor 1, Alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) ; Class E Basic Helix-Loop-Helix Protein 78; Basic-Helix-Loop-Helix-PAS Protein MOP1; Hypoxia-Inducible Factor 1-Alpha; PAS Domain-Containing Protein 8; Member Of PAS Protein 1; HIF-1-Alpha; BHLHe78; PASD8; MOP1; Hypoxia-Inducible Factor 1 Alpha Isoform I.3; Hypoxia Inducible Factor 1 Alpha Subunit; Hypoxia-Inducible Factor1alpha; Member Of PAS Superfamily 1; ARNT Interacting Protein; ARNT-Interacting Protein; HIF-1alpha; HIF1-ALPHA; HIF1-Alpha; BHLHE78; HIF-1A; HIF1
Background

Gene Name: HIF1A

NCBI Gene Entry: 3091

UniProt Entry: Q16665

Application Information

Molecular Weight: Predicted, 93 kDa, observed, 120 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1625

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human HIF-1-alpha
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using hypoxia inducible factor 1 subunit alpha antibody (Cat#9321). Total cell lysates (30 μg) from various cell lines untreated or treated with cobalt chloride (250 μM, 6 hr) were loaded and separated by SDS-PAGE. The blot was incubated with hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat.#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of hypoxia inducible factor 1 subunit alpha expression in HT-1080 cells using hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:2,000). Green, isotype control; red, hypoxia inducible factor 1 subunit alpha.
  • Immunocytochemical staining of HT-1080 cells with Hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:1,000). Nuclei were stained blue with DAPI; Hypoxia inducible factor 1 subunit alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    HIF1A
  • Catalog：
    9321
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using hypoxia inducible factor 1 subunit alpha antibody (Cat#9321). Total cell lysates (30 μg) from various cell lines untreated or treated with cobalt chloride (250 μM, 6 hr) were loaded and separated by SDS-PAGE. The blot was incubated with hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat.#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of hypoxia inducible factor 1 subunit alpha expression in HT-1080 cells using hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:2,000). Green, isotype control; red, hypoxia inducible factor 1 subunit alpha.
Immunocytochemical staining of HT-1080 cells with Hypoxia inducible factor 1 subunit alpha antibody (Cat#9321, 1:1,000). Nuclei were stained blue with DAPI; Hypoxia inducible factor 1 subunit alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
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  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

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    8:30 AM - 5:30 PM EST Mon-Fri

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  146. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_param.php ( 0.75 KB )
  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
  148. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/mobile.php ( 4.08 KB )
  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
  155. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/foot.php ( 5.05 KB )
  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  157. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.001666s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002153s ]
  3. SELECT * FROM `goods` WHERE `code` = '9321' LIMIT 1 [ RunTime:0.002056s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001273s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001344s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.002113s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 7045 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001387s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001140s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 7045 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001758s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.001037s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000864s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.180688s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8207,7838,4345,8605,9004,4532,8439,9817,5189,6086) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.004513s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.006172s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000250s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.005737s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000313s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001318s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.001080s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000259s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.001574s ]
0.309024s