Western blotting analysis using SRY-box transcription factor 2 antibody (Cat#H1124). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SRY-box transcription factor 2 antibody (Cat#H1124, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of SRY-box transcription factor 2 expression in A549 cells using SRY-box transcription factor 2 antibody (Cat#H1124, 1:2,000). Green, isotype control; red, SRY-box transcription factor 2.
Immunocytochemical staining shows positive staining in paraffin-embedded human stomach. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human lung. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human breast cancer. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human seminoma. SOX2 antibody (Cat#H1124) was used at 1:2,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Western blotting analysis using SRY-box transcription factor 2 antibody (Cat#H1124). SRY-box transcription factor 2 expression in wild-type (WT) and SRY-box transcription factor 2 (SOX2) knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SRY-box transcription factor 2 antibody (Cat#H1124, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
