Western blotting analysis using synaptophysin antibody (Cat#H1125). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with synaptophysin antibody (Cat#H1125, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Synaptophysin expression in A549 cells using Synaptophysin antibody (Cat#H1125, 1:2,000). Green, isotype control; red, Synaptophysin.
Immunocytochemical staining shows positive staining in paraffin-embedded human pancreas. Synaptophysin antibody (Cat#H1125) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded rat pancreas. Synaptophysin antibody (Cat#H1125) was used at 1:4,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human brain. Synaptophysin antibody (Cat#H1125) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon. Synaptophysin antibody (Cat#H1125) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human medullary thyroid carcinoma. Synaptophysin antibody (Cat#H1125) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human small cell lung carcinoma. Synaptophysin antibody (Cat#H1125) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human cervix cancer. Synaptophysin antibody (Cat#H1125) was used at 1:1,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded mouse brain. Synaptophysin antibody (Cat#H1125) was used at 1:4,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded mouse pancreas. Synaptophysin antibody (Cat#H1125) was used at 1:4,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded rat brain. Synaptophysin antibody (Cat#H1125) was used at 1:4,000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.Counterstained with hematoxylin.Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.