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Home>Product>Primary Antibody

KD-Validated RBFOX3 Recombinant Rabbit mAb

#H1138

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Western blotting analysis using RNA binding fox-1 homolog 3 antibody (Cat#H1138). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RNA binding fox-1 homolog 3 antibody (Cat#H1138). RNA binding fox-1 homolog 3 expression in wild-type (WT) and RNA binding fox-1 homolog 3 (RBFOX3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of RNA binding fox-1 homolog 3 expression in A549 cells using RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:2,000). Green, isotype control; red, RNA binding fox-1 homolog 3.
Immunocytochemical staining shows positive staining in paraffin-embedded human cerebellum. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunohistochemistry was performed on paraffin-embedded mouse brain using RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining shows negative staining in paraffin-embedded human cardiac muscle. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human glioma. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human baldder cancer. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded mouse cerebral cortex. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded mouse liver. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded rat cerebral cortex. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded rat liver. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
20 μL，100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    RBFOX3
  • Catalog：
    H1138
  • Application：
    WB, IHC-P, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated RBFOX3 Recombinant Rabbit mAb
Aliases RBFOX3; RNA Binding Fox-1 Homolog 3; HRNBP3; FOX-3; NeuN; Hexaribonucleotide Binding Protein 3; RNA Binding Protein, Fox-1 Homolog 3; RNA Binding Protein Fox-1 Homolog 3; Neuronal Nuclei Antigen ; Fox-1 Homolog C; NeuN Antigen; RNA Binding Protein, Fox-1 Homolog (C. Elegans) 3; Neuronal Nuclei; FOX3
Background

Gene Name: RBFOX3

NCBI Gene Entry: 146713

UniProt Entry: A6NFN3

Application Information

Molecular Weight: Predicted, 34 kDa, Observed, 55 kDa

Clonality: Recombinant Monoclonal Antibody

Clone ID: SDT-432-1

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human NeuN
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunohistochemistry-Paraffin (IHC-P): 1:500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using RNA binding fox-1 homolog 3 antibody (Cat#H1138). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using RNA binding fox-1 homolog 3 antibody (Cat#H1138). RNA binding fox-1 homolog 3 expression in wild-type (WT) and RNA binding fox-1 homolog 3 (RBFOX3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of RNA binding fox-1 homolog 3 expression in A549 cells using RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:2,000). Green, isotype control; red, RNA binding fox-1 homolog 3.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human cerebellum. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunohistochemistry was performed on paraffin-embedded mouse brain using RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining shows negative staining in paraffin-embedded human cardiac muscle. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human glioma. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows negative staining in paraffin-embedded human baldder cancer. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded mouse cerebral cortex. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows negative staining in paraffin-embedded mouse liver. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded rat cerebral cortex. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows negative staining in paraffin-embedded rat liver. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunohistochemistry was performed on paraffin-embedded human glioblastoma using RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    RBFOX3
  • Catalog：
    H1138
  • Application：
    WB, IHC-P, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using RNA binding fox-1 homolog 3 antibody (Cat#H1138). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using RNA binding fox-1 homolog 3 antibody (Cat#H1138). RNA binding fox-1 homolog 3 expression in wild-type (WT) and RNA binding fox-1 homolog 3 (RBFOX3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of RNA binding fox-1 homolog 3 expression in A549 cells using RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:2,000). Green, isotype control; red, RNA binding fox-1 homolog 3.
Immunocytochemical staining shows positive staining in paraffin-embedded human cerebellum. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunohistochemistry was performed on paraffin-embedded mouse brain using RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining shows negative staining in paraffin-embedded human cardiac muscle. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human glioma. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human baldder cancer. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded mouse cerebral cortex. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded mouse liver. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded rat cerebral cortex. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded rat liver. NeuN antibody (Cat#H1138) was used at 1:500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using RNA binding fox-1 homolog 3 antibody (Cat#H1138, 1:500). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
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  1. CONNECT:[ UseTime:0.000527s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000790s ]
  3. SELECT * FROM `goods` WHERE `code` = 'H1138' LIMIT 1 [ RunTime:0.000488s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000388s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000346s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000349s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 28 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000511s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000411s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 28 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000261s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000403s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000276s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.057692s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5153,8355,9326,9828,5494,5476,9431,9055,9813,4047) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000779s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000508s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000252s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000511s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000253s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000329s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000433s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000264s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000209s ]
0.095869s