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Home>Product>Primary Antibody

PRPF8 Recombinant Rabbit mAb

#1134

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Recombinant Antibody
Western blotting analysis using PRPF8 antibody (Cat#1134). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRPF8 antibody (Cat#1134, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PRPF8, pre-mRNA processing factor 8.
Immunocytochemical staining of HepG2 cells with PRPF8 antibody (Cat#1134, 1:1,000). Nuclei were stained blue with DAPI; PRPF8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PRPF8 expression in HepG2 cells using PRPF8 antibody (Cat#1134, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PRPF8.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PRPF8
  • Catalog：
    1134
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name PRPF8 Recombinant Rabbit mAb
Aliases PRPF8; Pre-MRNA Processing Factor 8; PRPC8; SNRNP220; Pre-MRNA-Processing-Splicing Factor 8; 220 KDa U5 SnRNP-Specific Protein; Splicing Factor Prp8; PRP8 Homolog; HPRP8; PRP8; RP13; P220; U5 SnRNP-Specific Protein (220 KD), Ortholog Of S. Cerevisiae Prp8p; PRP8 Pre-MRNA Processing Factor 8 Homolog (S. Cerevisiae); PRP8 Pre-MRNA Processing Factor 8 Homolog (Yeast); PRP8 Pre-MRNA Processing Factor 8 Homolog; Precursor MRNA Processing Protein; Apoptosis-Regulated Protein 1; Apoptosis-Regulated Protein 2; HPrp8; Prp8
Background

Gene Name: PRPF8

NCBI Gene Entry: 10594

UniProt Entry: Q6P2Q9

Application Information

Molecular Weight: Predicted, 274 kDa; observed, 274 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1995

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human PRPF8
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PRPF8 antibody (Cat#1134). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRPF8 antibody (Cat#1134, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PRPF8, pre-mRNA processing factor 8.
  • Immunocytochemical staining of HepG2 cells with PRPF8 antibody (Cat#1134, 1:1,000). Nuclei were stained blue with DAPI; PRPF8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of PRPF8 expression in HepG2 cells using PRPF8 antibody (Cat#1134, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PRPF8.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PRPF8
  • Catalog：
    1134
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using PRPF8 antibody (Cat#1134). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRPF8 antibody (Cat#1134, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PRPF8, pre-mRNA processing factor 8.
Immunocytochemical staining of HepG2 cells with PRPF8 antibody (Cat#1134, 1:1,000). Nuclei were stained blue with DAPI; PRPF8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PRPF8 expression in HepG2 cells using PRPF8 antibody (Cat#1134, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PRPF8.
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