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Home>Product>Primary Antibody

Cadherin 2 Recombinant Rabbit mAb

#1206

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Recombinant Antibody
Western blotting analysis using Cadherin 2 antibody (Cat#1206). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cadherin 2 antibody (Cat#1206, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using cadherin 2 antibody (Cat#1206, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Cadherin 2 expression in HepG2 cells using Cadherin 2 antibody (Cat#1206, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cadherin 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CDH2
  • Catalog：
    1206
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name Cadherin 2 Recombinant Rabbit mAb
Aliases CDH2; Cadherin 2; CDHN; CD325; NCAD; Cadherin 2, Type 1, N-Cadherin (Neuronal); Neural Cadherin; N-Cadherin; Cadherin-2; CDw325; Calcium-Dependent Adhesion Protein, Neuronal; CD325 Antigen; N-Cadherin 1; ARVD14; ACOGS; ADHD8
Background

Gene Name: CDH2

NCBI Gene Entry: 1000

UniProt Entry: P19022

Application Information

Molecular Weight: Predicted, 100 kDa; observed, 140 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2210

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human N Cadherin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using Cadherin 2 antibody (Cat#1206). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cadherin 2 antibody (Cat#1206, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using cadherin 2 antibody (Cat#1206, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of Cadherin 2 expression in HepG2 cells using Cadherin 2 antibody (Cat#1206, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cadherin 2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CDH2
  • Catalog：
    1206
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using Cadherin 2 antibody (Cat#1206). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cadherin 2 antibody (Cat#1206, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using cadherin 2 antibody (Cat#1206, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Cadherin 2 expression in HepG2 cells using Cadherin 2 antibody (Cat#1206, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Cadherin 2.
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