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Home>Product>Primary Antibody

Phospho-Raf1 (S259) Recombinant Rabbit mAb

#1278

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Recombinant Antibody
Western blotting analysis using Phospho-Raf1 (S259) antibody (Cat#1278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Raf1 (S259) antibody (Cat#1278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Phospho-Raf1 (S259) antibody (Cat#1278, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Raf1 (S259) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-Raf1 (S259) expression in HepG2 cells using Phospho-Raf1 (S259) antibody (Cat#1278, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-Raf1 (S259).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RAF1
  • Catalog：
    1278
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-Raf1 (S259) Recombinant Rabbit mAb
Aliases RAF1; Raf-1 Proto-Oncogene, Serine/Threonine Kinase; Raf-1; CRAF; RAF Proto-Oncogene Serine/Threonine-Protein Kinase; V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1; C-Raf Proto-Oncogene, Serine/Threonine Kinase; Proto-Oncogene C-RAF; EC 2.7.11.1; C-Raf; V-Raf-1 Murine Leukemia Viral Oncogene-Like Protein 1; Raf Proto-Oncogene Serine/Threonine Protein Kinase; Oncogene RAF1; EC 2.7.11; CMD1NN; C-RAF; RAF-1; CRaf; NS5; RAF
Background

Gene Name: RAF1

NCBI Gene Entry: 5894

UniProt Entry: P04049

Application Information

Molecular Weight: Predicted, 73 kDa; observed, 73 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2645

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-Raf1 (S259)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-Raf1 (S259) antibody (Cat#1278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Raf1 (S259) antibody (Cat#1278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Phospho-Raf1 (S259) antibody (Cat#1278, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Raf1 (S259) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-Raf1 (S259) expression in HepG2 cells using Phospho-Raf1 (S259) antibody (Cat#1278, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-Raf1 (S259).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RAF1
  • Catalog：
    1278
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Phospho-Raf1 (S259) antibody (Cat#1278). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Raf1 (S259) antibody (Cat#1278, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Phospho-Raf1 (S259) antibody (Cat#1278, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Raf1 (S259) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-Raf1 (S259) expression in HepG2 cells using Phospho-Raf1 (S259) antibody (Cat#1278, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-Raf1 (S259).
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