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Home>Product>Primary Antibody

Ring Finger Protein 20 Recombinant Rabbit mAb

#1306

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Recombinant Antibody
Western blotting analysis using Ring finger protein 20 antibody (Cat#1306). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Ring finger protein 20 antibody (Cat#1306, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Ring finger protein 20 antibody (Cat#1306, 1:1,000). Nuclei were stained blue with DAPI; Ring finger protein 20 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Ring finger protein 20 expression in HeLa cells using Ring finger protein 20 antibody (Cat#1306, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Ring finger protein 20.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RNF20
  • Catalog：
    1306
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name Ring Finger Protein 20 Recombinant Rabbit mAb
Aliases RNF20; Ring Finger Protein 20; BRE1A; HBRE1; BRE1; RING-Type E3 Ubiquitin Transferase BRE1A; E3 Ubiquitin-Protein Ligase BRE1A; FLJ20382; FLJ11189; KAIA2779; BRE1 E3 Ubiquitin Ligase Homolog (S. Cerevisiae); BRE1 E3 Ubiquitin Ligase Homolog; Homolog Of S. Cerevisiae BRE1; RING Finger Protein 20; EC 2.3.2.27; EC 6.3.2; BRE1-A
Background

Gene Name: RNF20

NCBI Gene Entry: 56254

UniProt Entry: Q5VTR2

Application Information

Molecular Weight: Predicted, 114 kDa; observed, 114 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2555

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human RNF20
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Ring finger protein 20 antibody (Cat#1306). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Ring finger protein 20 antibody (Cat#1306, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Ring finger protein 20 antibody (Cat#1306, 1:1,000). Nuclei were stained blue with DAPI; Ring finger protein 20 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Ring finger protein 20 expression in HeLa cells using Ring finger protein 20 antibody (Cat#1306, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Ring finger protein 20.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RNF20
  • Catalog：
    1306
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using Ring finger protein 20 antibody (Cat#1306). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Ring finger protein 20 antibody (Cat#1306, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Ring finger protein 20 antibody (Cat#1306, 1:1,000). Nuclei were stained blue with DAPI; Ring finger protein 20 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Ring finger protein 20 expression in HeLa cells using Ring finger protein 20 antibody (Cat#1306, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Ring finger protein 20.
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