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Home>Product>Primary Antibody

AKT1/AKT2/AKT3 Recombinant Rabbit mAb

#1366

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Recombinant Antibody
Western blotting analysis using AKT1/AKT2/AKT3 antibody (Cat#1366). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKT1/AKT2/AKT3 antibody (Cat#1366, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with AKT1/AKT2/AKT3 antibody (Cat#1366, 1:1,000). Nuclei were stained blue with DAPI; AKT1/AKT2/AKT3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of AKT1/AKT2/AKT3 expression in C2C12 cells using AKT1/AKT2/AKT3 antibody (Cat#1366, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AKT1/AKT2/AKT3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AKT1/2/3
  • Catalog：
    1366
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name AKT1/AKT2/AKT3 Recombinant Rabbit mAb
Aliases AKT1/2/3; AKT Serine/Threonine Kinase 1/2/3; RAC; PKB; PRKBA; AKT; V-Akt Murine Thymoma Viral Oncogene Homolog 1/2/3; RAC-Alpha Serine/Threonine-Protein Kinase; Protein Kinase B Alpha; Proto-Oncogene C-Akt; Protein Kinase B; RAC-PK-Alpha; EC 2.7.11.1; RAC-ALPHA; PKB Alpha; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1; Serine-Threonine Protein Kinase; Rac Protein Kinase Alpha; RAC-Alpha; PKB-ALPHA; EC 2.7.11; AKT1m
Background

Gene Name: AKT1/2/3

NCBI Gene Entry: 207 , 208 , 10000

UniProt Entry: P31749 , P31751 , Q9Y243

Application Information

Molecular Weight: Predicted, 56 kDa; observed, 56 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3180

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human AKT1 + AKT2 + AKT3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using AKT1/AKT2/AKT3 antibody (Cat#1366). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKT1/AKT2/AKT3 antibody (Cat#1366, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with AKT1/AKT2/AKT3 antibody (Cat#1366, 1:1,000). Nuclei were stained blue with DAPI; AKT1/AKT2/AKT3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of AKT1/AKT2/AKT3 expression in C2C12 cells using AKT1/AKT2/AKT3 antibody (Cat#1366, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AKT1/AKT2/AKT3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    AKT1/2/3
  • Catalog：
    1366
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using AKT1/AKT2/AKT3 antibody (Cat#1366). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKT1/AKT2/AKT3 antibody (Cat#1366, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with AKT1/AKT2/AKT3 antibody (Cat#1366, 1:1,000). Nuclei were stained blue with DAPI; AKT1/AKT2/AKT3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of AKT1/AKT2/AKT3 expression in C2C12 cells using AKT1/AKT2/AKT3 antibody (Cat#1366, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, AKT1/AKT2/AKT3.
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