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Home>Product>Primary Antibody

XPD Recombinant Rabbit mAb

#1611

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Recombinant Antibody
Western blotting analysis using XPD antibody (Cat#1611). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with XPD antibody (Cat#1611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with XPD antibody (Cat#1611, 1:1,000). Nuclei were stained blue with DAPI; XPD with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of XPD expression in HeLa cells using XPD antibody (Cat#1611, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, XPD.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ERCC2
  • Catalog：
    1611
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name XPD Recombinant Rabbit mAb
Aliases ERCC2; ERCC Excision Repair 2, TFIIH Core Complex Helicase Subunit; EM9; XPD; Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2; General Transcription And DNA Repair Factor IIH Helicase Subunit XPD; TFIIH Basal Transcription Factor Complex Helicase XPB Subunit; TFIIH Basal Transcription Factor Complex 80 KDa Subunit; Xeroderma Pigmentosum Group D-Complementing Protein; Excision Repair Cross-Complementation Group 2; Xeroderma Pigmentosum Complementary Group D; DNA Repair Protein Complementing XP-D Cells; Basic Transcription Factor 2 80 KDa Subunit; DNA Excision Repair Protein ERCC-2; TFIIH 80 KDa Subunit; TFIIH Subunit XPD; MGC102762; MGC126218; MGC126219; TFIIH P80; BTF2 P80; CXPD; MAG; Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2 Protein; TFIIH Basal Transcription Factor Complex Helicase XPD Subunit; TFIIH Basal Transcription Factor Complex Helicase Subunit; EC 3.6.4.12; EC 3.6.1; COFS2; TFIIH; TTD1; XPDC; TTD
Background

Gene Name: ERCC2

NCBI Gene Entry: 2068

UniProt Entry: P18074

Application Information

Molecular Weight: Predicted, 87 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1675

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human XPD
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using XPD antibody (Cat#1611). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with XPD antibody (Cat#1611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Hela cells with XPD antibody (Cat#1611, 1:1,000). Nuclei were stained blue with DAPI; XPD with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of XPD expression in HeLa cells using XPD antibody (Cat#1611, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, XPD.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ERCC2
  • Catalog：
    1611
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using XPD antibody (Cat#1611). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with XPD antibody (Cat#1611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with XPD antibody (Cat#1611, 1:1,000). Nuclei were stained blue with DAPI; XPD with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of XPD expression in HeLa cells using XPD antibody (Cat#1611, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, XPD.
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