Western blotting analysis using centrosomal protein 55 antibody (Cat#5804). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with centrosomal protein 55 antibody (Cat#5804, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Centrosomal protein 55 expression in HeLa cells using Centrosomal protein 55 antibody (Cat#5804, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Centrosomal protein 55.
CEP55; Centrosomal Protein 55; C10orf3; CT111; Up-Regulated In Colon Cancer 6; Centrosomal Protein Of 55 KDa; Cancer/Testis Antigen 111; Centrosomal Protein 55kDa; FLJ10540; URCC6; Chromosome 10 Open Reading Frame 3; MARCH; Cep55
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human CEP55
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using centrosomal protein 55 antibody (Cat#5804). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with centrosomal protein 55 antibody (Cat#5804, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Centrosomal protein 55 expression in HeLa cells using Centrosomal protein 55 antibody (Cat#5804, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Centrosomal protein 55.
Western blotting analysis using centrosomal protein 55 antibody (Cat#5804). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with centrosomal protein 55 antibody (Cat#5804, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Centrosomal protein 55 expression in HeLa cells using Centrosomal protein 55 antibody (Cat#5804, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Centrosomal protein 55.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
