Western blotting analysis using A-kinase anchoring protein 14 antibody (Cat#1652). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with A-kinase anchoring protein 14 antibody (Cat#1652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9C2 cells with A-kinase anchoring protein 14 antibody (Cat#1652, 1:1,000). Nuclei were stained blue with DAPI; A-kinase anchoring protein 14 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of A-kinase anchoring protein 14 expression in H9c2 cells using A-kinase anchoring protein 14 antibody (Cat#1652, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, A-kinase anchoring protein 14.
A-Kinase Anchoring Protein 14 Recombinant Rabbit mAb
Aliases
AKAP14; A-Kinase Anchoring Protein 14; AKAP28; Protein Kinase A-Anchoring Protein 14; A Kinase (PRKA) Anchor Protein 14; A-Kinase Anchor Protein 28 KDa; A-Kinase Anchor Protein 14; AKAP 28; AKAP-14; PRKA14; A-Kinase Anchoring Protein 28
Western blotting analysis using A-kinase anchoring protein 14 antibody (Cat#1652). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with A-kinase anchoring protein 14 antibody (Cat#1652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9C2 cells with A-kinase anchoring protein 14 antibody (Cat#1652, 1:1,000). Nuclei were stained blue with DAPI; A-kinase anchoring protein 14 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of A-kinase anchoring protein 14 expression in H9c2 cells using A-kinase anchoring protein 14 antibody (Cat#1652, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, A-kinase anchoring protein 14.
Western blotting analysis using A-kinase anchoring protein 14 antibody (Cat#1652). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with A-kinase anchoring protein 14 antibody (Cat#1652, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of H9C2 cells with A-kinase anchoring protein 14 antibody (Cat#1652, 1:1,000). Nuclei were stained blue with DAPI; A-kinase anchoring protein 14 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of A-kinase anchoring protein 14 expression in H9c2 cells using A-kinase anchoring protein 14 antibody (Cat#1652, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, A-kinase anchoring protein 14.
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