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Home>Product>Primary Antibody

Phospho-RSK1 p90 (T573) Recombinant Rabbit mAb

#1698

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Recombinant Antibody
Western blotting analysis using Phospho-RSK1 p90 (T573) antibody (Cat#1698). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-RSK1 p90 (T573) antibody (Cat#1698, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Phospho-RSK1 p90 (T573) antibody (Cat#1698, 1:1,000). Nuclei were stained blue with DAPI; Phospho-RSK1 p90 (T573) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-RSK1 p90 (T573) expression in C2C12 cells using Phospho-RSK1 p90 (T573) antibody (Cat#1698, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-RSK1 p90 (T573).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KA1
  • Catalog：
    1698
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-RSK1 p90 (T573) Recombinant Rabbit mAb
Aliases RPS6KA1; Ribosomal Protein S6 Kinase A1; RSK1; MAPKAPK1; HU-1; MAP Kinase-Activated Protein Kinase 1a; 90 KDa Ribosomal Protein S6 Kinase 1; Ribosomal Protein S6 Kinase Alpha-1; MAPK-Activated Protein Kinase 1a; Ribosomal S6 Kinase 1; MAPKAP Kinase 1a; EC 2.7.11.1; MAPKAPK-1a; MAPKAPK1A; P90-RSK 1; P90RSK1; P90Rsk; P90S6K; RSK-1; DJ590P13.1 (Ribosomal Protein S6 Kinase, 90kD, Polypeptide 1); Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1; Ribosomal Protein S6 Kinase, 90kD, Polypeptide 1; S6K-Alpha 1; S6K-Alpha-1; EC 2.7.11; P90RSK; RSK
Background

Gene Name: RPS6KA1

NCBI Gene Entry: 6195

UniProt Entry: Q15418

Application Information

Molecular Weight: Predicted, 83 kDa; observed, 83 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4130

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Phospho-RSK1 p90 (T573)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-RSK1 p90 (T573) antibody (Cat#1698). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-RSK1 p90 (T573) antibody (Cat#1698, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with Phospho-RSK1 p90 (T573) antibody (Cat#1698, 1:1,000). Nuclei were stained blue with DAPI; Phospho-RSK1 p90 (T573) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Phospho-RSK1 p90 (T573) expression in C2C12 cells using Phospho-RSK1 p90 (T573) antibody (Cat#1698, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-RSK1 p90 (T573).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RPS6KA1
  • Catalog：
    1698
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Phospho-RSK1 p90 (T573) antibody (Cat#1698). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-RSK1 p90 (T573) antibody (Cat#1698, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Phospho-RSK1 p90 (T573) antibody (Cat#1698, 1:1,000). Nuclei were stained blue with DAPI; Phospho-RSK1 p90 (T573) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-RSK1 p90 (T573) expression in C2C12 cells using Phospho-RSK1 p90 (T573) antibody (Cat#1698, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-RSK1 p90 (T573).
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