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Home>Product>Primary Antibody

Phospho-ERK1 (T202/Y204) + ERK2 (T185/Y187) Recombinant Rabbit mAb

#1796

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Recombinant Antibody
Western blotting analysis using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (20 ug) from various breast cancer cell lines were loaded and separated by SDS-PAGE. The membrane was incubated with the respective primary antibody and corresponding HRP-conjugated secondary antibody. Images were developed using Clarity Western ECL Substrate. For reference, we use the Bio-Rad Dual Protein Ladder and the provided ladder images are merged colorimetric. Included for each image is expected observed protein sizes, the antibody dilution, and chemiluminescent exposure time.
Flow cytometric analysis of Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in C2C12 cells using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAPK3/1
  • Catalog：
    1796
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Phospho-ERK1 (T202/Y204) + ERK2 (T185/Y187) Recombinant Rabbit mAb
Aliases MAPK3; Mitogen-Activated Protein Kinase 3; ERK1; PRKM3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; EC 2.7.11.24; P44-ERK1; P44-MAPK; P44ERK1; P44MAPK; ERK-1; ERT2; Extracellular Signal-Related Kinase 1; MAP Kinase Isoform P44; MAP Kinase; EC 2.7.11; HS44KDAP; HUMKER1A; P44mapk; P44erk1; MAPK 1; MAPK 3; MAPK1; Mitogen-Activated Protein Kinase 1; ERK2; Extracellular Signal-Regulated Kinase 2; MAPK2; PRKM1; PRKM2; ERK; Mitogen-Activated Protein Kinase 2; MAP Kinase 1; MAP Kinase 2; EC 2.7.11.24; P42-MAPK; P41mapk; MAPK; ERK-2; ERT1; Protein Tyrosine Kinase; ERK2; MAP Kinase Isoform P42; EC 2.7.11; P42MAPK; P41MAPK; MAPK 1; NS13; P38; P40; P41
Background

Gene Name: MAPK3/1

NCBI Gene Entry: 5595 , 5594

UniProt Entry: P27361 , P28482

Application Information

Molecular Weight: Predicted, 43,41 kDa; observed, 42,44 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4505

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human Phospho-ERK1 (Y204) + ERK2 (Y187)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (20 ug) from various breast cancer cell lines were loaded and separated by SDS-PAGE. The membrane was incubated with the respective primary antibody and corresponding HRP-conjugated secondary antibody. Images were developed using Clarity Western ECL Substrate. For reference, we use the Bio-Rad Dual Protein Ladder and the provided ladder images are merged colorimetric. Included for each image is expected observed protein sizes, the antibody dilution, and chemiluminescent exposure time.
  • Flow cytometric analysis of Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in C2C12 cells using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAPK3/1
  • Catalog：
    1796
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796). Total cell lysates (20 ug) from various breast cancer cell lines were loaded and separated by SDS-PAGE. The membrane was incubated with the respective primary antibody and corresponding HRP-conjugated secondary antibody. Images were developed using Clarity Western ECL Substrate. For reference, we use the Bio-Rad Dual Protein Ladder and the provided ladder images are merged colorimetric. Included for each image is expected observed protein sizes, the antibody dilution, and chemiluminescent exposure time.
Flow cytometric analysis of Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in C2C12 cells using Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) antibody (Cat#1796, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187).
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