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Home>Product>Primary Antibody

ATP5A1 Recombinant Rabbit mAb

#1896

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Recombinant Antibody
Western blotting analysis using ATP5A1 antibody (Cat#1896). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP5A1 antibody (Cat#1896, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ATP5A1 antibody (Cat#1896, 1:1,000). Nuclei were stained blue with DAPI; ATP5A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ATP5A1 expression in HepG2 cells using ATP5A1 antibody (Cat#1896, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP5A1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP5F1A
  • Catalog：
    1896
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name ATP5A1 Recombinant Rabbit mAb
Aliases ATP5F1A; ATP Synthase F1 Subunit Alpha; ATP5A; ATP5AL2; ATP5A1; HATP1; ATPM; OMR; ORM; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Alpha Subunit 1, Cardiac Muscle; ATP Synthase Subunit Alpha, Mitochondrial; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Alpha Subunit, Isoform 2, Non-Cardiac Muscle-Like 2; ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, Alpha Subunit, Isoform 1, Cardiac Muscle; Epididymis Secretory Sperm Binding Protein Li 123m; Mitochondrial ATP Synthetase, Oligomycin-Resistant; ATP Synthase Alpha Chain, Mitochondrial; ATP Sythase (F1-ATPase) Alpha Subunit; EC 3.6.3.14; HEL-S-123m; EC 3.6.3; COXPD22; MC5DN4A; MC5DN4B; MC5DN4; MOM2
Background

Gene Name: ATP5F1A

NCBI Gene Entry: 498

UniProt Entry: P25705

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 54 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4850

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human ATP5A1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ATP5A1 antibody (Cat#1896). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP5A1 antibody (Cat#1896, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with ATP5A1 antibody (Cat#1896, 1:1,000). Nuclei were stained blue with DAPI; ATP5A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ATP5A1 expression in HepG2 cells using ATP5A1 antibody (Cat#1896, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP5A1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP5F1A
  • Catalog：
    1896
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ATP5A1 antibody (Cat#1896). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP5A1 antibody (Cat#1896, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ATP5A1 antibody (Cat#1896, 1:1,000). Nuclei were stained blue with DAPI; ATP5A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ATP5A1 expression in HepG2 cells using ATP5A1 antibody (Cat#1896, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP5A1.
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