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Solute Carrier Family 6 Member 1 Recombinant Rabbit mAb

#2666

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Recombinant Antibody
Western blotting analysis using Solute carrier family 6 member 1 antibody (Cat#2666). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Solute carrier family 6 member 1 antibody (Cat#2666, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Solute carrier family 6 member 1 antibody (Cat#2666, 1:1,000). Nuclei were stained blue with DAPI; Solute carrier family 6 member 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Solute carrier family 6 member 1 expression in C2C12 cells using Solute carrier family 6 member 1 antibody (Cat#2666, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Solute carrier family 6 member 1.
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Unit price:
$69.00
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  • Gene Symbol:
    SLC6A1
  • Catalog:
    2666
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    M
Information
Product Name Solute Carrier Family 6 Member 1 Recombinant Rabbit mAb
Aliases SLC6A1; Solute Carrier Family 6 Member 1; GABATR; GAT1; Sodium- And Chloride-Dependent GABA Transporter 1; GABATHG; HGAT-1; GAT-1; Solute Carrier Family 6 (Neurotransmitter Transporter, GABA), Member 1; Solute Carrier Family 6 (Neurotransmitter Transporter), Member 1; GABA Transporter 1; GABT1; MAE
Background

Gene Name: SLC6A1

NCBI Gene Entry: 6529

UniProt Entry: P30531

Application Information

Molecular Weight: Predicted, 67 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2390

Species Reactivity: Mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human SLC6A1 / GAT1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Solute carrier family 6 member 1 antibody (Cat#2666). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Solute carrier family 6 member 1 antibody (Cat#2666, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with Solute carrier family 6 member 1 antibody (Cat#2666, 1:1,000). Nuclei were stained blue with DAPI; Solute carrier family 6 member 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Solute carrier family 6 member 1 expression in C2C12 cells using Solute carrier family 6 member 1 antibody (Cat#2666, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Solute carrier family 6 member 1.
Western blotting analysis using Solute carrier family 6 member 1 antibody (Cat#2666). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Solute carrier family 6 member 1 antibody (Cat#2666, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Solute carrier family 6 member 1 antibody (Cat#2666, 1:1,000). Nuclei were stained blue with DAPI; Solute carrier family 6 member 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Solute carrier family 6 member 1 expression in C2C12 cells using Solute carrier family 6 member 1 antibody (Cat#2666, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Solute carrier family 6 member 1.
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