Western blotting analysis using FHL2 antibody (Cat#2072). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FHL2 antibody (Cat#2072, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with FHL2 antibody (Cat#2072, 1:1,000). Nuclei were stained blue with DAPI; FHL2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of FHL2 expression in HT-1080 cells using FHL2 antibody (Cat#2072, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FHL2.
FHL2; Four And A Half LIM Domains 2; SLIM3; DRAL; Four And A Half LIM Domains Protein; Skeletal Muscle LIM-Protein 3; LIM Domain Protein DRAL; SLIM-3; FHL-2; Down-Regulated In Rhabdomyosarcoma LIM Protein; Aging-Associated Gene 11; AAG11
Western blotting analysis using FHL2 antibody (Cat#2072). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FHL2 antibody (Cat#2072, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with FHL2 antibody (Cat#2072, 1:1,000). Nuclei were stained blue with DAPI; FHL2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of FHL2 expression in HT-1080 cells using FHL2 antibody (Cat#2072, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FHL2.
Western blotting analysis using FHL2 antibody (Cat#2072). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FHL2 antibody (Cat#2072, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with FHL2 antibody (Cat#2072, 1:1,000). Nuclei were stained blue with DAPI; FHL2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of FHL2 expression in HT-1080 cells using FHL2 antibody (Cat#2072, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, FHL2.
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