Western blotting analysis using ACSS2 antibody (Cat#69177). Total cell lysates (45 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ACSS2 antibody (Cat#69177, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1: 20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). ACSS2, acyl-CoA synthetase short chain family member 2.
Western blotting analysis using ACSS2 antibody (Cat#69177). ACSS2 expression in wild type (WT) and ACSS2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with ACSS2 antibody (Cat#69177, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of ACSS2 expression in HepG2 cells using ACSS2 antibody (Cat#69177, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ACSS2.