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Home>Product>Primary Antibody

Histone H2B (Formyl K120) Recombinant Rabbit mAb

#2089

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Recombinant Antibody
Western blotting analysis using Histone H2B (formyl K120) antibody (Cat#2089). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone H2B (formyl K120) antibody (Cat#2089, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Histone H2B (formyl K120) antibody (Cat#2089, 1:1,000). Nuclei were stained blue with DAPI; Histone H2B (formyl K120) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    H2BC21
  • Catalog：
    2089
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Histone H2B (Formyl K120) Recombinant Rabbit mAb
Aliases H2BC21; H2B Clustered Histone 21; H2B-GL105; HIST2H2BE; H2B/Q; H2B.1; H2BFQ; H2BE; Histone Cluster 2 H2B Family Member E; H2B Histone Family, Member Q; Histone Cluster 2, H2be; Histone H2B Type 2-E; Histone H2B-GL105; Histone 2, H2be; Histone H2B.Q; H2B; H2B-Clustered Histone 21; H2BGL105; GL105; H2BQ
Background

Gene Name: H2BC21

NCBI Gene Entry: 8349

UniProt Entry: Q16778

Application Information

Molecular Weight: Predicted, 14 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5945

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Histone H2B (formyl K120)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Histone H2B (formyl K120) antibody (Cat#2089). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone H2B (formyl K120) antibody (Cat#2089, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with Histone H2B (formyl K120) antibody (Cat#2089, 1:1,000). Nuclei were stained blue with DAPI; Histone H2B (formyl K120) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    H2BC21
  • Catalog：
    2089
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Histone H2B (formyl K120) antibody (Cat#2089). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Histone H2B (formyl K120) antibody (Cat#2089, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Histone H2B (formyl K120) antibody (Cat#2089, 1:1,000). Nuclei were stained blue with DAPI; Histone H2B (formyl K120) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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