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Glycophorin C Recombinant Rabbit mAb

#2182

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Recombinant Antibody
Immunocytochemical staining of HAP-1 cells with Glycophorin C antibody (Cat#2182, 1:1,000). Nuclei were stained blue with DAPI; Glycophorin C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using Glycophorin C antibody (Cat#2182). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Glycophorin C antibody (Cat#2182, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    GYPC
  • Catalog:
    2182
  • Application:
    WB, ICC
  • Species Reactivity:
    H
Information
Product Name Glycophorin C Recombinant Rabbit mAb
Aliases GYPC; Glycophorin C (Gerbich Blood Group); GPC; Sialoglycoprotein D; CD236R; CD236; GYPD; GPD; Glycoprotein Beta; Gerbich Antigen; Glycoconnectin; Glycophorin-C; Glycophorin-D; PAS-2'; GE; Gerbich Blood Group Antigen; Glycophorin D; CD236 Antigen; PAS-2; GLPC; Ge
Background

Gene Name: GYPC

NCBI Gene Entry: 2995

UniProt Entry: P04921

Application Information

Molecular Weight: Predicted, 14 kDa; observed, 39 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6390

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human Glycophorin C
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Immunocytochemical staining of HAP-1 cells with Glycophorin C antibody (Cat#2182, 1:1,000). Nuclei were stained blue with DAPI; Glycophorin C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Western blotting analysis using Glycophorin C antibody (Cat#2182). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Glycophorin C antibody (Cat#2182, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Glycophorin C antibody (Cat#2182, 1:1,000). Nuclei were stained blue with DAPI; Glycophorin C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using Glycophorin C antibody (Cat#2182). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Glycophorin C antibody (Cat#2182, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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