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Home>Product>Primary Antibody

Rho A + B + C Recombinant Rabbit mAb

#2440

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Recombinant Antibody
Western blotting analysis using Rho A + B + C antibody (Cat#2440). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Rho A + B + C antibody (Cat#2440, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Rho A + B + C antibody (Cat#2440, 1:1,000). Nuclei were stained blue with DAPI; Rho A + B + C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Rho A + B + C expression in C2C12 cells using Rho A + B + C antibody (Cat#2440, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Rho A + B + C.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RHOA/RHOB/RHOC
  • Catalog：
    2440
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Rho A + B + C Recombinant Rabbit mAb
Aliases Ras Homolog Family Member A; RHOH12; Rho12; ARH12; ARHA; Transforming Protein RhoA; Epididymis Secretory Sperm Binding Protein; Ras Homolog Gene Family, Member A; Aplysia Ras-Related Homolog 12; Small GTP Binding Protein RhoA; Rho CDNA Clone 12; Oncogene RHO H12; EC 3.6.5.2; EDFAOB; RHO12; H12; Ras Homolog Family Member B; MST081; RHOH6; ARH6; ARHB; Rho-Related GTP-Binding Protein RhoB; Ras Homolog Gene Family, Member B; Rho CDNA Clone 6; Oncogene RHO H6; H6; Aplysia RAS-Related Homolog 6; MSTP081; Ras Homolog Family Member C; ARH9; ARHC; Rho-Related GTP-Binding Protein RhoC; Ras Homolog Gene Family, Member C; Rho CDNA Clone 9; H9; Epididymis Secretory Sperm Binding Protein; Small GTP Binding Protein RhoC; RAS-Related Homolog 9; Oncogene RHO H9; RhoC GTPase; RHOH9
Background

Gene Name: RHOA/RHOB/RHOC

NCBI Gene Entry: 387 , 388 , 389

UniProt Entry: P61586 , P62745 , P08134

Application Information

Molecular Weight: Predicted, 22 kDa; observed, 22 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB850

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Rho A + B + C
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Rho A + B + C antibody (Cat#2440). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Rho A + B + C antibody (Cat#2440, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with Rho A + B + C antibody (Cat#2440, 1:1,000). Nuclei were stained blue with DAPI; Rho A + B + C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Rho A + B + C expression in C2C12 cells using Rho A + B + C antibody (Cat#2440, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Rho A + B + C.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RHOA/RHOB/RHOC
  • Catalog：
    2440
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Rho A + B + C antibody (Cat#2440). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Rho A + B + C antibody (Cat#2440, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Rho A + B + C antibody (Cat#2440, 1:1,000). Nuclei were stained blue with DAPI; Rho A + B + C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Rho A + B + C expression in C2C12 cells using Rho A + B + C antibody (Cat#2440, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Rho A + B + C.
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