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PKC Alpha + Beta + Gamma Recombinant Rabbit mAb

#2447

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Recombinant Antibody
Western blotting analysis using PKC alpha + beta + gamma antibody (Cat#2447). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PKC alpha + beta + gamma antibody (Cat#2447, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with PKC alpha + beta + gamma antibody (Cat#2447, 1:1,000). Nuclei were stained blue with DAPI; PKC alpha + beta + gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PKC alpha + beta + gamma expression in C2C12 cells using PKC alpha + beta + gamma antibody (Cat#2447, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PKC alpha + beta + gamma.
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  • Gene Symbol:
    PRKCB/PRKCA/PRKCG/PRKCE
  • Catalog:
    2447
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name PKC Alpha + Beta + Gamma Recombinant Rabbit mAb
Aliases PRKCA ; Protein Kinase C Alpha; PKCA; Protein Kinase C Alpha Type; EC 2.7.11.13; PKC-Alpha; PRKACA; PKC-A; Protein Kinase C, Alpha; Aging-Associated Gene 6; EC 2.7.11; PKCalpha; PKCI+/-; PKCα; AAG6; PRKCB ; Protein Kinase C Beta; PRKCB1; PKCB; Protein Kinase C Beta Type; EC 2.7.11.13; PKC-Beta; PRKCB2; PKC-B; Protein Kinase C, Beta 1 Polypeptide; Protein Kinase C, Beta 1; Protein Kinase C, Beta; EC 2.7.11; PKCI(2); PKCbeta; PKCβ; PRKCE; Protein Kinase C Epsilon; Protein Kinase C Epsilon Type; NPKC-Epsilon; EC 2.7.11.13; PKCE; Protein Kinase C, Epsilon; EC 2.7.11; PRKCG; Protein Kinase C Gamma; PKC-Gamma; PKCC; PKCG; Protein Kinase C Gamma Type; EC 2.7.11.13; MGC57564; SCA14; Protein Kinase C, Gamma; EC 2.7.11; PKCgamma; PKCI(3); PKCγ
Background

Gene Name: PRKCB/PRKCA/PRKCG/PRKCE

NCBI Gene Entry: 5579 , 5578 , 5582 , 5581

UniProt Entry: P05771 , P17252 , P05129 , Q02156

Application Information

Molecular Weight: Predicted, 77,78 kDa; observed, 77 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB885

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human PKC
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PKC alpha + beta + gamma antibody (Cat#2447). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PKC alpha + beta + gamma antibody (Cat#2447, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with PKC alpha + beta + gamma antibody (Cat#2447, 1:1,000). Nuclei were stained blue with DAPI; PKC alpha + beta + gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of PKC alpha + beta + gamma expression in C2C12 cells using PKC alpha + beta + gamma antibody (Cat#2447, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PKC alpha + beta + gamma.
Western blotting analysis using PKC alpha + beta + gamma antibody (Cat#2447). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PKC alpha + beta + gamma antibody (Cat#2447, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with PKC alpha + beta + gamma antibody (Cat#2447, 1:1,000). Nuclei were stained blue with DAPI; PKC alpha + beta + gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of PKC alpha + beta + gamma expression in C2C12 cells using PKC alpha + beta + gamma antibody (Cat#2447, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PKC alpha + beta + gamma.
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