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Home>Product>Primary Antibody

RNA Sensor RIG-I Recombinant Rabbit mAb

#2512

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Recombinant Antibody
Western blotting analysis using RIGI antibody (Cat#2512). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RIGI antibody (Cat#2512, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with RIGI antibody (Cat#2512, 1:1,000). Nuclei were stained blue with DAPI; RIGI was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RIGI expression in HT-1080 cells using RIGI antibody (Cat#2512, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RIGI.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RIGI
  • Catalog：
    2512
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name RNA Sensor RIG-I Recombinant Rabbit mAb
Aliases RNA Sensor RIG-I; RIG-I; Antiviral Innate Immune Response Receptor RIG-I; RIG-1; DDX58; RIG1; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58; Retinoic Acid-Inducible Gene 1 Protein; Retinoic Acid-Inducible Gene I Protein; ATP-Dependent RNA Helicase DDX58; DExD/H-Box Helicase 58; DEAD Box Protein 58; RNA Helicase RIG-I; DKFZp434J1111; FLJ13599; RLR-1; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide; Probable ATP-Dependent RNA Helicase DDX58; Retinoic Acid Inducible Gene I; RIG-I-Like Receptor 1; EC 3.6.4.13; SGMRT2
Background

Gene Name: RIGI

NCBI Gene Entry: 23586

UniProt Entry: O95786

Application Information

Molecular Weight: Predicted, 107 kDa; observed, 107 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1195

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human DDX58
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using RIGI antibody (Cat#2512). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RIGI antibody (Cat#2512, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with RIGI antibody (Cat#2512, 1:1,000). Nuclei were stained blue with DAPI; RIGI was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of RIGI expression in HT-1080 cells using RIGI antibody (Cat#2512, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RIGI.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    RIGI
  • Catalog：
    2512
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using RIGI antibody (Cat#2512). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RIGI antibody (Cat#2512, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with RIGI antibody (Cat#2512, 1:1,000). Nuclei were stained blue with DAPI; RIGI was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of RIGI expression in HT-1080 cells using RIGI antibody (Cat#2512, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, RIGI.
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