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Podoplanin Recombinant Rabbit mAb

#2568

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Recombinant Antibody
Western blotting analysis using Podoplanin antibody (Cat#2568). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Podoplanin antibody (Cat#2568, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Podoplanin expression in C2C12 cells using Podoplanin antibody (Cat#2568, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Podoplanin.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PDPN
  • Catalog:
    2568
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M
Information
Product Name Podoplanin Recombinant Rabbit mAb
Aliases PDPN; Podoplanin; Aggrus; PA2.26; T1A-2; D2-40; Gp38; GP40; Lung Type I Cell Membrane Associated Glycoprotein; Glycoprotein 36; PA2.26 Antigen;
T1-Alpha; GP36; T1A; Lung Type-I Cell Membrane-Associated Glycoprotein (T1A-2); Glycoprotein, 36-KD; HT1alpha-1; HT1alpha-2; HT1A-1; OTS8; T1A2;
TI1A; Gp36
Background

Gene Name: PDPN

NCBI Gene Entry: 10630

UniProt Entry: Q86YL7

Application Information

Molecular Weight: Predicted, 17 kDa; observed, 38 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1440

Species Reactivity: Human,mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human Podoplanin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using Podoplanin antibody (Cat#2568). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Podoplanin antibody (Cat#2568, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Podoplanin expression in C2C12 cells using Podoplanin antibody (Cat#2568, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Podoplanin.
Western blotting analysis using Podoplanin antibody (Cat#2568). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Podoplanin antibody (Cat#2568, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Podoplanin expression in C2C12 cells using Podoplanin antibody (Cat#2568, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Podoplanin.
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