Western blotting analysis using Phospho-Protein Kinase D2 (S876) antibody (Cat#2617). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-Protein Kinase D2 (S876) antibody (Cat#2617, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with Phospho-Protein Kinase D2 (S876) antibody (Cat#2617, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Protein Kinase D2 (S876) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Phospho-Protein Kinase D2 (S876) expression in HAP-1 cells using Phospho-Protein Kinase D2 (S876) antibody (Cat#2617, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Phospho-Protein Kinase D2 (S876).
