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SRSF1 Recombinant Rabbit mAb

#2656

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Recombinant Antibody
Western blotting analysis using SRSF1 antibody (Cat#2656). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SRSF1 antibody (Cat#2656, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of SRSF1 expression in HepG2 cells using SRSF1 antibody (Cat#2656, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SRSF1.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    SRSF1
  • Catalog:
    2656
  • Application:
    WB, FCM
  • Species Reactivity:
    H, M, R
Information
Product Name SRSF1 Recombinant Rabbit mAb
Aliases SRSF1; Serine And Arginine Rich Splicing Factor 1; ASF; SF2; Pre-MRNA-Splicing Factor SF2, P33 Subunit; Serine/Arginine-Rich Splicing Factor 1; SRp30a; SF2p33; SFRS1; Splicing Factor, Arginine/Serine-Rich 1; Alternative-Splicing Factor 1; SR Splicing Factor 1; Splicing Factor 2; MGC5228; Splicing Factor, Arginine/Serine-Rich, 30-KD, A; Alternate Splicing Factor; NEDFBA; SF2P33; ASF-1
Background

Gene Name: SRSF1

NCBI Gene Entry: 6426

UniProt Entry: Q07955

Application Information

Molecular Weight: Predicted, 28 kDa; observed, 33 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2255

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human SF2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using SRSF1 antibody (Cat#2656). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SRSF1 antibody (Cat#2656, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of SRSF1 expression in HepG2 cells using SRSF1 antibody (Cat#2656, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SRSF1.
Western blotting analysis using SRSF1 antibody (Cat#2656). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SRSF1 antibody (Cat#2656, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of SRSF1 expression in HepG2 cells using SRSF1 antibody (Cat#2656, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, SRSF1.
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