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Home>Product>Primary Antibody

KD-Validated ATP1B3 Recombinant Rabbit mAb

#61848

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using ATP1B3 antibody (Cat#61848). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP1B3 antibody (Cat#61848, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ATP1B3 antibody (Cat#61848). ATP1B3 expression in wild type (WT) and ATP1B3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP1B3 antibody (Cat#61848, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP1B3
  • Catalog：
    61848
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated ATP1B3 Recombinant Rabbit mAb
Aliases ATP1B3; ATPase Na+/K+ Transporting Subunit Beta 3; Sodium/Potassium-Transporting ATPase Subunit Beta-3; CD298; Sodium-Potassium ATPase Subunit Beta 3 (Non-Catalytic); Sodium/Potassium-Dependent ATPase Subunit Beta-3; ATPase, Na+/K+ Transporting, Beta 3 Polypeptide; Sodium Pump Subunit Beta-3; FLJ29027; ATPB-3; Sodium/Potassium-Transporting ATPase Beta-3 Chain; Na, K-ATPase Beta-3 Polypeptide; CD298 Antigen
Background

Gene Name: ATP1B3

NCBI Gene Entry: 483

UniProt Entry: P54709

Application Information

Molecular Weight: Predicted, 32 kDa; observed, 37 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4740

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human ATP1B3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using ATP1B3 antibody (Cat#61848). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP1B3 antibody (Cat#61848, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using ATP1B3 antibody (Cat#61848). ATP1B3 expression in wild type (WT) and ATP1B3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP1B3 antibody (Cat#61848, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ATP1B3
  • Catalog：
    61848
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ATP1B3 antibody (Cat#61848). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP1B3 antibody (Cat#61848, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using ATP1B3 antibody (Cat#61848). ATP1B3 expression in wild type (WT) and ATP1B3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP1B3 antibody (Cat#61848, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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