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OXSM Mouse mAb

#5912

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Western blotting analysis using OXSM antibody (Cat#5912). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with OXSM antibody (Cat#5912, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with OXSM antibody(Cat#5912, 1:1,000). Nuclei were stained blue with DAPI; OXSM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    OXSM
  • Catalog:
    5912
  • Application:
    WB, ICC
  • Species Reactivity:
    H
Information
Product Name OXSM Mouse mAb
Aliases OXSM; 3-Oxoacyl-ACP Synthase, Mitochondrial; FASN2D; CEM1; KS; 3-Oxoacyl-[Acyl-Carrier-Protein] Synthase, Mitochondrial; Beta-Ketoacyl-ACP Synthase; Beta-Ketoacyl Synthase; EC 2.3.1.41; FLJ20604; Acyl-Malonyl Acyl Carrier Protein-Condensing Enzyme; Type II Mitochondrial Beta-Ketoacyl Synthase; 3-Ketoacyl-Acyl Carrier Protein Synthase; 3-Oxoacyl-Acyl-Carrier-Protein Synthase; EC 2.3.1; KASI
Background

Gene Name: OXSM

NCBI Gene Entry: 54995

UniProt Entry: Q9NWU1

Application Information

Molecular Weight: Predicted, 49 kDa; observed, 46 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5795

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen Recombinant protein of human OXSM
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using OXSM antibody (Cat#5912). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with OXSM antibody (Cat#5912, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with OXSM antibody(Cat#5912, 1:1,000). Nuclei were stained blue with DAPI; OXSM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Western blotting analysis using OXSM antibody (Cat#5912). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with OXSM antibody (Cat#5912, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with OXSM antibody(Cat#5912, 1:1,000). Nuclei were stained blue with DAPI; OXSM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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