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Home>Product>Primary Antibody

SMARCB1 Recombinant Rabbit mAb

#2757

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Recombinant Antibody
Western blotting analysis using SMARCB1 antibody (Cat#2757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCB1 antibody (Cat#2757, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with SMARCB1 antibody (Cat#2757, 1:1,000). Nuclei were stained blue with DAPI; SMARCB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SMARCB1 expression in HAP-1 cells using SMARCB1 antibody (Cat#2757, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMARCB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMARCB1
  • Catalog：
    2757
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name SMARCB1 Recombinant Rabbit mAb
Aliases SMARCB1; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily B, Member 1; BAF47; PPP1R144; SNF5L1; INI-1; HSNFS; Sfh1p; Ini1; Snr1; SNF5; RDT; SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator Of Chromatin Subfamily B Member 1; Protein Phosphatase 1, Regulatory Subunit 144; Sucrose Nonfermenting, Yeast, Homolog-Like 1; Malignant Rhabdoid Tumor Suppressor; Integrase Interactor 1 Protein; BRG1-Associated Factor 47; SNF5 Homolog; HSNF5; SWI/SNF-Related Matrix-Associated Protein; Integrase Interactor 1; SWNTS1; MRD15; RTPS1; CSS3; INI1
Background

Gene Name: SMARCB1

NCBI Gene Entry: 6598

UniProt Entry: Q12824

Application Information

Molecular Weight: Predicted, 44 kDa; observed, 44 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1935

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human SNF5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SMARCB1 antibody (Cat#2757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCB1 antibody (Cat#2757, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with SMARCB1 antibody (Cat#2757, 1:1,000). Nuclei were stained blue with DAPI; SMARCB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of SMARCB1 expression in HAP-1 cells using SMARCB1 antibody (Cat#2757, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMARCB1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SMARCB1
  • Catalog：
    2757
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using SMARCB1 antibody (Cat#2757). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCB1 antibody (Cat#2757, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with SMARCB1 antibody (Cat#2757, 1:1,000). Nuclei were stained blue with DAPI; SMARCB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of SMARCB1 expression in HAP-1 cells using SMARCB1 antibody (Cat#2757, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, SMARCB1.
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