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Home>Product>Primary Antibody

Thioredoxin Domain Containing 12 Recombinant Rabbit mAb

#2801

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Recombinant Antibody
Western blotting analysis using thioredoxin domain containing 12 antibody (Cat#2801). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with thioredoxin domain containing 12 antibody (Cat#2801, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Thioredoxin domain containing 12 antibody (Cat#2801, 1:1,000). Nuclei were stained blue with DAPI; Thioredoxin domain containing 12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Thioredoxin domain containing 12 expression in C2C12 cells using Thioredoxin domain containing 12 antibody (Cat#2801, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Thioredoxin domain containing 12.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    TXNDC12
  • Catalog：
    2801
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Thioredoxin Domain Containing 12 Recombinant Rabbit mAb
Aliases TXNDC12; Thioredoxin Domain Containing 12; TLP19; PDIA16; ERP18; ERP19; HAG-1; AGR1; Endoplasmic Reticulum Thioredoxin Superfamily Member, 18 KDa; Thioredoxin Domain Containing 12 (Endoplasmic Reticulum); Protein Disulfide Isomerase Family A, Member 16; Endoplasmic Reticulum Resident Protein 18; Endoplasmic Reticulum Resident Protein 19; Thioredoxin Domain-Containing Protein 12; Thioredoxin-Like Protein P19; ER Protein 18; ER Protein 19; HTLP19; Anterior Gradient Homolog 1 (Xenopus Laevis); Endoplasmic Reticulum Protein ERp19; Anterior Gradient Homolog 1; EC 1.8.4.2; ERP16; ERp18; ERp19; AG1
Background

Gene Name: TXNDC12

NCBI Gene Entry: 51060

UniProt Entry: O95881

Application Information

Molecular Weight: Predicted, 19 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1835

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human ERp19
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using thioredoxin domain containing 12 antibody (Cat#2801). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with thioredoxin domain containing 12 antibody (Cat#2801, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with Thioredoxin domain containing 12 antibody (Cat#2801, 1:1,000). Nuclei were stained blue with DAPI; Thioredoxin domain containing 12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Thioredoxin domain containing 12 expression in C2C12 cells using Thioredoxin domain containing 12 antibody (Cat#2801, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Thioredoxin domain containing 12.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TXNDC12
  • Catalog：
    2801
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using thioredoxin domain containing 12 antibody (Cat#2801). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with thioredoxin domain containing 12 antibody (Cat#2801, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Thioredoxin domain containing 12 antibody (Cat#2801, 1:1,000). Nuclei were stained blue with DAPI; Thioredoxin domain containing 12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Thioredoxin domain containing 12 expression in C2C12 cells using Thioredoxin domain containing 12 antibody (Cat#2801, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Thioredoxin domain containing 12.
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