Western blotting analysis using dysferlin antibody (Cat#2933). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dysferlin antibody (Cat#2933, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse skeletal muscle using dysferlin antibody (Cat#2933, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Dysferlin expression in HT-1080 cells using Dysferlin antibody (Cat#2933, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Dysferlin.
Western blotting analysis using dysferlin antibody (Cat#2933). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dysferlin antibody (Cat#2933, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse skeletal muscle using dysferlin antibody (Cat#2933, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Dysferlin expression in HT-1080 cells using Dysferlin antibody (Cat#2933, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Dysferlin.
Western blotting analysis using dysferlin antibody (Cat#2933). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dysferlin antibody (Cat#2933, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse skeletal muscle using dysferlin antibody (Cat#2933, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of Dysferlin expression in HT-1080 cells using Dysferlin antibody (Cat#2933, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Dysferlin.
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