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Home>Product>Primary Antibody

ARAF Recombinant Rabbit mAb

#2956

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Recombinant Antibody
Western blotting analysis using ARAF antibody (Cat#2956). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARAF antibody (Cat#2956, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ARAF antibody (Cat#2956, 1:1,000). Nuclei were stained blue with DAPI; ARAF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ARAF expression in HepG2 cells using ARAF antibody (Cat#2956, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ARAF.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ARAF
  • Catalog：
    2956
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name ARAF Recombinant Rabbit mAb
Aliases ARAF; A-Raf Proto-Oncogene, Serine/Threonine Kinase; ARAF1; V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog 1; Serine/Threonine-Protein Kinase A-Raf; Proto-Oncogene A-Raf-1; Proto-Oncogene Pks; EC 2.7.11.1; A-Raf; PKS2; V-Raf Murine Sarcoma 3611 Viral Oncogene-Like Protein; A-Raf Proto-Oncogene Serine/Threonine-Protein Kinase; Ras-Binding Protein DA-Raf; Proto-Oncogene A-Raf; Oncogene ARAF1; EC 2.7.11; RAFA1; PKS
Background

Gene Name: ARAF

NCBI Gene Entry: 369

UniProt Entry: P10398

Application Information

Molecular Weight: Predicted, 68 kDa; observed, 68 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3345

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human A-RAF
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ARAF antibody (Cat#2956). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARAF antibody (Cat#2956, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with ARAF antibody (Cat#2956, 1:1,000). Nuclei were stained blue with DAPI; ARAF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ARAF expression in HepG2 cells using ARAF antibody (Cat#2956, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ARAF.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ARAF
  • Catalog：
    2956
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using ARAF antibody (Cat#2956). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARAF antibody (Cat#2956, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with ARAF antibody (Cat#2956, 1:1,000). Nuclei were stained blue with DAPI; ARAF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ARAF expression in HepG2 cells using ARAF antibody (Cat#2956, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ARAF.
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