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Home>Product>Primary Antibody

Natriuretic Peptide Receptor 3 Recombinant Rabbit mAb

#3004

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Recombinant Antibody
Western blotting analysis using Natriuretic peptide receptor 3 antibody (Cat#3004). Total cell lysates (15 μg for C2C12 and H9c2, 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Natriuretic peptide receptor 3 antibody (Cat#3004, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Natriuretic peptide receptor 3 antibody (Cat#3004, 1:1,000). Nuclei were stained blue with DAPI; Natriuretic peptide receptor 3 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Natriuretic peptide receptor 3 expression in C2C12 cells using Natriuretic peptide receptor 3 antibody (Cat#3004, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,Natriuretic peptide receptor 3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NPR3
  • Catalog：
    3004
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Natriuretic Peptide Receptor 3 Recombinant Rabbit mAb
Aliases NPR3; Natriuretic Peptide Receptor 3; C5orf23; GUCY2B; ANPRC; NPRC; Natriuretic Peptide Receptor C/Guanylate Cyclase C (Atrionatriuretic Peptide Receptor C); Atrial Natriuretic Peptide Clearance Receptor; Atrial Natriuretic Peptide Receptor Type C; Atrial Natriuretic Peptide Receptor 3; Atrionatriuretic Peptide Receptor C; Guanylate Cyclase C; FLJ14054; ANPR-C; ANP-C; NPR-C; Chromosome 5 Open Reading Frame 23; Natriuretic Peptide Receptor C; BOMOS
Background

Gene Name: NPR3

NCBI Gene Entry: 4883

UniProt Entry: P17342

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB3585

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Natriuretic Peptide Receptor C
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using Natriuretic peptide receptor 3 antibody (Cat#3004). Total cell lysates (15 μg for C2C12 and H9c2, 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Natriuretic peptide receptor 3 antibody (Cat#3004, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of C2C12 cells with Natriuretic peptide receptor 3 antibody (Cat#3004, 1:1,000). Nuclei were stained blue with DAPI; Natriuretic peptide receptor 3 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Natriuretic peptide receptor 3 expression in C2C12 cells using Natriuretic peptide receptor 3 antibody (Cat#3004, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,Natriuretic peptide receptor 3.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NPR3
  • Catalog：
    3004
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using Natriuretic peptide receptor 3 antibody (Cat#3004). Total cell lysates (15 μg for C2C12 and H9c2, 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Natriuretic peptide receptor 3 antibody (Cat#3004, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of C2C12 cells with Natriuretic peptide receptor 3 antibody (Cat#3004, 1:1,000). Nuclei were stained blue with DAPI; Natriuretic peptide receptor 3 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Natriuretic peptide receptor 3 expression in C2C12 cells using Natriuretic peptide receptor 3 antibody (Cat#3004, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red,Natriuretic peptide receptor 3.
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