KD-Validated Caspase 9 Recombinant Rabbit mAb - Product

KD-Validated Caspase 9 Recombinant Rabbit mAb

#63154

Click to copy product information

Return to List

Recombinant Antibody
shRNA Knockdown Validated

Western blotting analysis using caspase 9 antibody (Cat#63154). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 9 antibody (Cat#63154, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using caspase 9 antibody (Cat#63154). Caspase 9 expression in wild type (WT) and caspase 9 (CASP9) shRNA knockdown (KD) HeLa cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with caspase 9 antibody (Cat#63154, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Flow cytometric analysis of caspase 9 expression in HepG2 cells using caspase 9 antibody (Cat#63154, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, caspase 9.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

CASP9
Catalog：

63154
Application：

WB, FCM
Species Reactivity：

H

Information

Data

Information

Product Name	KD-Validated Caspase 9 Recombinant Rabbit mAb
Aliases	Caspase 9; ICE-LAP6; APAF-3; MCH6; PPP1R56; Caspase 9, Apoptosis-Related Cysteine Peptidase; Protein Phosphatase 1, Regulatory Subunit 56; ICE-Like Apoptotic Protease 6; Caspase-9; Caspase 9, Apoptosis-Related Cysteine Protease; Apoptotic Protease Activating Factor 3; Apoptotic Protease-Activating Factor 3; Apoptotic Protease MCH-6; Apoptotic Protease Mch-6; EC 3.4.22.62; CASP-9; APAF3
Background	Gene Name: CASP9 NCBI Gene Entry: 842 UniProt Entry: P55211
Application Information	Molecular Weight: Predicted, 46 kDa; observed, 46 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB4275 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human Caspase-9
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using caspase 9 antibody (Cat#63154). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 9 antibody (Cat#63154, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using caspase 9 antibody (Cat#63154). Caspase 9 expression in wild type (WT) and caspase 9 (CASP9) shRNA knockdown (KD) HeLa cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with caspase 9 antibody (Cat#63154, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of caspase 9 expression in HepG2 cells using caspase 9 antibody (Cat#63154, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, caspase 9.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

CASP9
Catalog：

63154
Application：

WB, FCM
Species Reactivity：

H

Datasheet

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News