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TATA-Box Binding Protein Like 1 Recombinant Rabbit mAb

#3099

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Recombinant Antibody
Western blotting analysis using TATA-box binding protein like 1 antibody (Cat#3099). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TATA-box binding protein like 1 antibody (Cat#3099, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with TATA-box binding protein like 1 antibody (Cat#3099, 1:1,000). Nuclei were stained blue with DAPI; TATA-box binding protein like 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TATA-box binding protein like 1 expression in HT-1080 cells using TATA-box binding protein like 1 antibody (Cat#3099, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TATA-box binding protein like 1.
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Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    TBPL1
  • Catalog:
    3099
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, R
Information
Product Name TATA-Box Binding Protein Like 1 Recombinant Rabbit mAb
Aliases TBPL; TATA-Box Binding Protein Like 1; STUD; TRF2; TLP; TLF; TBP-Like 1; TATA Box-Binding Protein-Related Factor 2; Second TBP Of Unique DNA Protein; TATA Box-Binding Protein-Like 1; TBP-Related Factor 2; TBP-Related Protein; TBP-Like Factor;
TATA Box-Binding Protein-Like Protein 1; 21-KDA TBP-Like Protein; 21 KDa TBP-Like Protein; MGC:8389; MGC:9620; TLP21; TRP
Background

Gene Name: TBPL1

NCBI Gene Entry: 9519

UniProt Entry: P62380

Application Information

Molecular Weight: Predicted, 21 kDa; observed, 23 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4060

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human TBPL1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using TATA-box binding protein like 1 antibody (Cat#3099). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TATA-box binding protein like 1 antibody (Cat#3099, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HT-1080 cells with TATA-box binding protein like 1 antibody (Cat#3099, 1:1,000). Nuclei were stained blue with DAPI; TATA-box binding protein like 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of TATA-box binding protein like 1 expression in HT-1080 cells using TATA-box binding protein like 1 antibody (Cat#3099, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TATA-box binding protein like 1.
Western blotting analysis using TATA-box binding protein like 1 antibody (Cat#3099). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TATA-box binding protein like 1 antibody (Cat#3099, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with TATA-box binding protein like 1 antibody (Cat#3099, 1:1,000). Nuclei were stained blue with DAPI; TATA-box binding protein like 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TATA-box binding protein like 1 expression in HT-1080 cells using TATA-box binding protein like 1 antibody (Cat#3099, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TATA-box binding protein like 1.
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