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Home>Product>Primary Antibody

Caspase 9 Recombinant Rabbit mAb

#3153

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Recombinant Antibody
Western blotting analysis using caspase 9 antibody (Cat#3153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 9 antibody (Cat#3153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Caspase 9 antibody (Cat#3153, 1:1,000). Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Caspase 9 expression in C2C12 cells using Caspase 9 antibody (Cat#3153, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Caspase 9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CASP9
  • Catalog：
    3153
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    M, R

Information

Data

Information
Product Name Caspase 9 Recombinant Rabbit mAb
Aliases Caspase 9; ICE-LAP6; APAF-3; MCH6; PPP1R56; Caspase 9, Apoptosis-Related Cysteine Peptidase; Protein Phosphatase 1, Regulatory Subunit 56; ICE-Like Apoptotic Protease 6; Caspase-9; Caspase 9, Apoptosis-Related Cysteine Protease; Apoptotic Protease Activating Factor 3; Apoptotic Protease-Activating Factor 3; Apoptotic Protease MCH-6; Apoptotic Protease Mch-6; EC 3.4.22.62; CASP-9; APAF3
Background

Gene Name: CASP9

NCBI Gene Entry: 842

UniProt Entry: P55211

Application Information

Molecular Weight: Predicted, 50 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4270

Species Reactivity: Mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of Mouse Caspase-9(UniProt Entry: Q8C3Q9)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using caspase 9 antibody (Cat#3153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 9 antibody (Cat#3153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with Caspase 9 antibody (Cat#3153, 1:1,000). Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Caspase 9 expression in C2C12 cells using Caspase 9 antibody (Cat#3153, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Caspase 9.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CASP9
  • Catalog：
    3153
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    M, R
RELATED PRODUCTS
Western blotting analysis using caspase 9 antibody (Cat#3153). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 9 antibody (Cat#3153, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Caspase 9 antibody (Cat#3153, 1:1,000). Nuclei were stained blue with DAPI; Caspase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Caspase 9 expression in C2C12 cells using Caspase 9 antibody (Cat#3153, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Caspase 9.
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