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Home>Product>Primary Antibody

Histone H4 (Acetyl K5) Recombinant Rabbit mAb

#5229

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Recombinant Antibody
Western blotting analysis using acetyl-Histone H4 (Lys5) antibody (Cat#5229). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with acetyl-Histone H4 (Lys5) antibody (Cat#5229, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Acetyl-Histone H4 (Lys5) antibody (Cat#5229, 1:1,000). Nuclei were stained blue with DAPI; Acetyl-Histone H4 (Lys5) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Acetyl-Histone H4 (Lys5) expression in C2C12 cells using Acetyl-Histone H4 (Lys5) antibody (Cat#5229, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Acetyl-Histone H4 (Lys5).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    H4C3
  • Catalog：
    5229
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Histone H4 (Acetyl K5) Recombinant Rabbit mAb
Aliases H4C3; H4 Clustered Histone 3; H4/G; DJ221C16.1; HIST1H4C; H4FG; Histone Cluster 1 H4 Family Member C; H4 Histone Family, Member G; Histone Cluster 1, H4c; Histone 1, H4c; Histone H4; H4-16; TEBIVANED1; TEVANED1; HIST1H4A; HIST1H4B; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST2H4; HIST4H4; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; H4C1; H4C2; H4C4; H4C5; H4C6; H4C8; H4C9; H4/A; H4FA; H4/I; H4FI; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH; H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; H4/O; H4FO
Background

Gene Name: H4C3

NCBI Gene Entry: 8364

UniProt Entry: P62805

Application Information

Molecular Weight: Predicted, 11 kDa; observed, 14 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB260

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human Histone H4 (acetyl K5)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using acetyl-Histone H4 (Lys5) antibody (Cat#5229). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with acetyl-Histone H4 (Lys5) antibody (Cat#5229, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with Acetyl-Histone H4 (Lys5) antibody (Cat#5229, 1:1,000). Nuclei were stained blue with DAPI; Acetyl-Histone H4 (Lys5) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Acetyl-Histone H4 (Lys5) expression in C2C12 cells using Acetyl-Histone H4 (Lys5) antibody (Cat#5229, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Acetyl-Histone H4 (Lys5).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    H4C3
  • Catalog：
    5229
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using acetyl-Histone H4 (Lys5) antibody (Cat#5229). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with acetyl-Histone H4 (Lys5) antibody (Cat#5229, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Acetyl-Histone H4 (Lys5) antibody (Cat#5229, 1:1,000). Nuclei were stained blue with DAPI; Acetyl-Histone H4 (Lys5) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Acetyl-Histone H4 (Lys5) expression in C2C12 cells using Acetyl-Histone H4 (Lys5) antibody (Cat#5229, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Acetyl-Histone H4 (Lys5).
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