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Home>Product>Primary Antibody

KD-Validated KDM3B Recombinant Rabbit mAb

#67022

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using KDM3B antibody (Cat#67022, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using KDM3B antibody (Cat#67022, 1:1,000). KDM3B expression in wild-type (WT) and KDM3B shRNA knockdown (KD) HeLa cells of total cell lysates. Hsp60 serves as a loading control.
Western blotting analysis using KDM3B antibody (Cat#67022). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KDM3B antibody (Cat#67022, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively.
Immunocytochemical staining of HeLa cells using anti-KDM3B antibody (Cat#67022, 1:100). Nuclei were stained blue with DAPI; β-actin (ACTB) was stained green with Alexa Flour® 488; KDM3B was stained magenta with Alexa Flour® 647.
Flow cytometric analysis of KDM3B expression in HeLa cells using KDM3B antibody (Cat#67022, 1:150). Red, isotype control; blue, KDM3B.
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Unit price：
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  • Gene Symbol：
    KDM3B
  • Catalog：
    67022
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, Mk

Information

Data

Information
Product Name KD-Validated KDM3B Recombinant Rabbit mAb
Aliases KDM3B; Lysine Demethylase 3B; KIAA1082; C5orf7; JMJD1B; NET22; JmjC Domain-Containing Histone Demethylation Protein 2B; [Histone H3]-Dimethyl-L-Lysine(9) Demethylase 3B; Jumonji Domain-Containing Protein 1B; Lysine (K)-Specific Demethylase 3B; Lysine-Specific Demethylase 3B; Jumonji Domain Containing 1B; Nuclear Protein 5qNCA; Chromosome 5 Open Reading Frame 7; EC 1.14.11.65; EC 1.14.11; JHDM2B; 5qNCA; DIJOS
Background

Gene Name: KDM3B

NCBI Gene Entry: 51780

UniProt Entry: Q7LBC6

Application Information

Molecular Weight: Predicted, 192 kDa; observed, 220 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB2430

Species Reactivity: Human, monkey

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human KDM3B
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000
Flow cytometry (FCM): 1:150
Immunocytochemistry (ICC): 1:100
Note This product is for research use only.
Data
  • Western blotting analysis using KDM3B antibody (Cat#67022, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
  • Western blotting analysis using KDM3B antibody (Cat#67022, 1:1,000). KDM3B expression in wild-type (WT) and KDM3B shRNA knockdown (KD) HeLa cells of total cell lysates. Hsp60 serves as a loading control.
  • Western blotting analysis using KDM3B antibody (Cat#67022). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KDM3B antibody (Cat#67022, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively.
  • Immunocytochemical staining of HeLa cells using anti-KDM3B antibody (Cat#67022, 1:100). Nuclei were stained blue with DAPI; β-actin (ACTB) was stained green with Alexa Flour® 488; KDM3B was stained magenta with Alexa Flour® 647.
  • Flow cytometric analysis of KDM3B expression in HeLa cells using KDM3B antibody (Cat#67022, 1:150). Red, isotype control; blue, KDM3B.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KDM3B
  • Catalog：
    67022
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, Mk
RELATED PRODUCTS
Western blotting analysis using KDM3B antibody (Cat#67022, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using KDM3B antibody (Cat#67022, 1:1,000). KDM3B expression in wild-type (WT) and KDM3B shRNA knockdown (KD) HeLa cells of total cell lysates. Hsp60 serves as a loading control.
Western blotting analysis using KDM3B antibody (Cat#67022). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KDM3B antibody (Cat#67022, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively.
Immunocytochemical staining of HeLa cells using anti-KDM3B antibody (Cat#67022, 1:100). Nuclei were stained blue with DAPI; β-actin (ACTB) was stained green with Alexa Flour® 488; KDM3B was stained magenta with Alexa Flour® 647.
Flow cytometric analysis of KDM3B expression in HeLa cells using KDM3B antibody (Cat#67022, 1:150). Red, isotype control; blue, KDM3B.
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