Western blotting analysis using phospho-EGFR (Tyr1068) antibody (Cat#3177). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-EGFR (Tyr1068) antibody (Cat#3177, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-EGFR (Tyr1068) antibody (Cat#3177) antibody. Total cell lysates (20 ug) from various breast cancer cell lines were loaded and separated by SDS-PAGE. The membrane was incubated with the respective primary antibody and corresponding HRP-conjugated secondary antibody. Images were developed using Clarity Western ECL Substrate. For reference, we use the Bio-Rad Dual Protein Ladder and the provided ladder images are merged colorimetric. Included for each image is expected observed protein sizes, the antibody dilution, and chemiluminescent exposure time.
Flow cytometric analysis of phospho-EGFR (Tyr1068) expression in HepG2 cells using phospho-EGFR (Tyr1068) antibody (Cat#3177, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, phospho-EGFR (Tyr1068).
