Western blotting analysis using ATP1A1 antibody (Cat#3340). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP1A1 antibody (Cat#3340, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using ATP1A1 antibody (Cat#3340, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with ATP1A1 antibody (Cat#3340, 1:1,000). Nuclei were stained blue with DAPI; ATP1A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Meduim. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Immunohistochemistry image of 4% PFA fixed, frozen, OCT-embedded adult mouse cerebellum tissue using Atp1a1 antibody (Cat#3340) at dilution 1:400. Atp1a1 (in magenta) was detected using 568 goat anti-rabbit IgG H-L, Highly-Cross-Adsorbed Secondary Antibody at dilution 1:400, and nuclei (blue) was detected using DAPI. Scalebar = 50 μm.Image courtesy of Monica D Sietam from Miguel Holmgren lab, the National Institute of Neurological Disorders and Stroke (NINDS), the National Institutes of Health (NIH). As a work of the U.S. Government, the image itself is not subject to copyright protection in the US. Image provided for educational purposes and does not represent an endorsement by NINDS, NIH, or the U.S. Government of any products or services for any purposes offered by the company.
Flow cytometric analysis of ATP1A1 expression in HepG2 cells using ATP1A1 antibody (Cat#3340, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ATP1A1.
