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FGR Recombinant Rabbit mAb

#5429

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Recombinant Antibody
Western blotting analysis using FGR antibody (Cat#5429). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FGR antibody (Cat#5429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of FGR expression in Raji cells using FGR antibody (Cat#5429, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, FGR.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    FGR
  • Catalog:
    5429
  • Application:
    WB, FCM
  • Species Reactivity:
    H
Information
Product Name FGR Recombinant Rabbit mAb
Aliases FGR; FGR Proto-Oncogene, Src Family Tyrosine Kinase; Gardner-Rasheed Feline Sarcoma Viral (V-Fgr) Oncogene Homolog; P55c-Fgr; C-Fgr; SRC2; V-Fgr Feline Gardner-Rasheed Sarcoma Viral Oncogene Homolog; Feline Gardner-Rasheed Sarcoma Viral Oncogene Homolog; Tyrosine-Protein Kinase Fgr; Proto-Oncogene C-Fgr; EC 2.7.10.2; P58c-Fgr; P55-Fgr; P58-Fgr; Proto-Oncogene Tyrosine-Protein Kinase FGR; C-Src-2 Proto-Oncogene; C-Fgr Protooncogene; P55-C-Fgr Protein; EC 2.7.10; C-Src2
Background

Gene Name: FGR

NCBI Gene Entry: 2268

UniProt Entry: P09769

Application Information

Molecular Weight: Predicted, 59 kDa; observed, 56 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB1510

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human FGR
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using FGR antibody (Cat#5429). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FGR antibody (Cat#5429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of FGR expression in Raji cells using FGR antibody (Cat#5429, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, FGR.
Western blotting analysis using FGR antibody (Cat#5429). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FGR antibody (Cat#5429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of FGR expression in Raji cells using FGR antibody (Cat#5429, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, FGR.
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